The simultaneous production of carbonic anhydrase and the phycobiliproteins, C-phycocyanin and allophycocyanin, was evaluated during the cultivation of Spirulina platensis using Zarrouk's medium in its non-diluted (100% ZM) and diluted to 20% (v/v) (20% ZM) forms. In both media, Spirulina simultaneously produced carbonic anhydrase and phycobiliproteins. For the phycobiliproteins' production, higher concentrations (mg/g) of C-phycocyanin and allophycocyanin were obtained when 100% ZM was used. For carbonic anhydrase production, higher concentrations (U/g) were obtained on the 6(th) day of cultivation in both culture media. 100% ZM promoted greater productions of these bioproducts, and, for simultaneous obtainment, the cells should be harvested after 21 days, obtaining concentrations of 137.4mg/g C-phycocyanin, 104.4mg/g allophycocyanin, and 26.6U/g carbonic anhydrase.