Temperature-dependent vibrational circular dichroism (VCD) measurements were performed in situ during the solgel transformation of a chiral low-molecular weight gelator, N,N'-diperfluoroheptanoyl-1,2(R,R)- or -1,2(S,S)-diaminocyclohexane in CD3CN. No VCD signal was detected for an isotropic acetonitrile solution of the gelator above 45 degrees C, while clear peaks appeared on the onset of gelation below 45 degrees C. They were assigned to the C=O stretching, NH bending and stretching vibrations related to perfluoroalkyl groups. The signal enhancement induced by gelation was ascribed to the occurrence of intermolecular vibrational coupling among the gelator molecules asymmetrically arranged within a fibril.