Cantharellus tubaeformis was processed by pressurized hot water extraction (80-240 degrees C) with the aim of maximizing the extraction of oligomeric fractions, -glucans, and the in vitro antioxidant properties of the extracts. Increased severity of treatment enhanced the extraction yields above 62% at temperatures of 210oC or higher, corresponding to the maximum -glucan yields. The highest antioxidant capacity was obtained at 170oC, although the highest content of phenolic compounds was obtained at the highest severity studied. This hydrothermal treatment can be considered a suitable process to obtain extracts with antioxidant properties and rich in -glucans.