Post injection is a multiple-injection strategy that is commonly used as a particulate matter control measure to reduce soot emissions, yet the mechanisms and the interactions between the main and post injections are only vaguely understood. For this work, experiments were performed to assess the effects of varying dwell time between the main and post injections in a compression-ignition (CI) engine environment simulated using a constant-volume combustion chamber. The ambient density, bulk temperature, and oxygen concentration used for this work were controlled at 19.4 kg/m(3), 900 K, and 15 vol % O-2, respectively. A canola oil-based biodiesel was tested and injected at a fixed injection pressure of 100 MPa into the simulated CI engine environment. A mass ratio of 80%-20% was maintained between the main and post injections, with the dwell time between the injections varied from 1.5 to 2.5 ms. Comparative measurements were performed using the same fuel and injection schedules, but at a higher ambient gas temperature condition of 1100 K. Optical diagnostics methods, including diffused-back illumination and high-speed flame luminosity imaging, were used to assess the spray and combustion processes of the post injection test case. Under the conditions of this work, it was found that the ignition delays, ignition locations, and flame lift-off lengths of the post injection flames are consistently shorter than those of the main injections, with the variations influenced by the extent of the interaction of the post injection with the combustion products from the main injection. A two-color pyrometry technique was also used to measure the soot temperature and soot concentration factor information on the main-post injection cases. The data revealed a greater interaction between the main and post injections resulted in a more rapid development of the soot zone of the post injection with higher temperature after ignition. The distribution of the most probable soot concentration factors of the post injection was also found to be narrower, with lower soot content values.