Despite the broad variety of substrates tested for lactic acid production, most of them have problems, even those with promising results. The use of sugar cane molasses appears as a viable alternative, allowing the development of high-performance lactic acid production with yeast extract. In this study, different strategies are proposed and evaluated to achieve high yield, productivity, and lactic acid concentrations considering the suitable association among strain physiology, osmotic stress, substrates, pH control, and fermentation operational parameters. With these strategies and using molasses as a substrate, it was possible to obtain a yield of 95%, with a productivity of 5.38 g L-1 h(-1), and a titer of 157.95 g/L of lactic acid.