Coalbed methane has emerged as a viable natural gas resource in India since 2007. The understanding of gas genesis kinetics, storage mechanisms and the geological controls is vital for exploration and successful recovery of gas in a cost-effective manner. In this respect, a multidisciplinary analytical approach including gas content, stable isotopes (delta C-13(1)), hydrocarbons distribution, reconstruction of original organic matter, chemical properties, sorption kinetics and the role of petrographic constituents have been assessed. The volatile matter content varies from 20.2 to 32.1 wt%; indicating medium volatile to high volatile bituminous rank of coal with the maximum vitrinite reflectance (VRo%) ranging from 0.63 to 0.98%. The studied coals are vitrinite rich and contains vitrinite group macerals in the range of 41-65 vol%. Dendritic micro-fractures are confined to vitrite microlithotype, and secondary mineralization helps preserve fracture connectivity. The average gas content of coal seams is 2.06 cc/g. The increase in gas content values per 100 m is about 0.38 cc/g emphasizing escape and migration of hydrocarbons during restructuring and tectonic activities in the basin. The relationship of C3/C1 and C2/C1 ratios is indicating that the hydrocarbons in desorbed gas originated from the thermogenic process. The large concentration of methane in desorbed gas and its stable isotope signatures (delta C-13(1) < -40 parts per thousand) also indicates the thermogenic origin of gases in coal seams. Few desorbed gas samples have been found in the mixed gas region, which may be due to the influx of fresh water carrying bacteria leading to the generation of biogenic gases and subsequent mixing with the sorbed thermogenic gases. The values of S1 (0.19-0.78 mg HC/g) and S2 (10.31-25.63 mg HC/g) indicate excellent source rock potentials. The positive correlation of original hydrogen index (HIO) with present-day hydrogen index (HIPD) and original organic content (TOCO) with present-day organic content (TOCPD) suggests uniform consistency in organic carbon conversion to hydrocarbons. The gas content compared with sorption capacity reveals the undersaturation of coal seams. The experimental results summarize that the low gas content is a critical issue. Similarly, the low, quality and quantity of CH4 may affect the exploration and recovery of methane in the long term into this basin. But, the low values of sorption time (0.12-6.65 days) signifies good diffusion characteristics that may support the recovery of gas. Finally, it is concluded that depth of occurrence, maturity and pore/cleats associated with microconstituents control the gas accumulation and transport in coal seam reservoirs of South Karanpura coalfield.