Experimental investigation on the TiO2 nanofluids jet impingement heat transfer and flow characteristics in the micro-channel heat sink are carried out. In the present study, three heat transfer enhancement techniques; micro-channel heat sink, jet impingement, and nanofluids are considered in which included the effect of relevant parameters of the nanofluids concentration, nozzle diameter, nozzle-to-heat sink distances, mass flow rate of nanofluids on the heat transfer performance of a micro-channel heat sink are considered. The obtained results showed that the suspending of nanoparticles in the base fluid remarkably increases the convective heat transfer by 18.56% at 0.015% nanofluids concentration. In addition, the obtained heat transfer coefficient tends to increase with increasing the nozzle diameter and decreasing nozzle level height. While the pressure drop across the test section increases as the nozzle diameter decreases and nozzle level height increases. However, the suspending of nanoparticles bring almost no extra addition of pressure drop as comparing with the base fluid. However, the obtained results point out that the proper selection of the relevant parameters to enhancement of heat transfer is important. (C) 2018 Elsevier Ltd. All rights reserved.