Bimetallic nanoporous alloys have attracted considerable attention for electrochemical sensor applications due to their excellent electrocatalytic activity and structural stability. In the present work, nanoporous PtFe (NP-PtFe) alloy was fabricated by chemical dealloying ternary PtFeAl alloy ribbons. The structure and morphology of NP-PtFe alloy was characterized by scanning electronmicroscopy, transmission electron microscopy and X-ray diffraction. A highly sensitive and selective biosensor based on NP-PtFe alloy and graphene was successfully developed for direct detection of bisphenol A. Under optimal conditions, the proposed sensor showed an efficient electrocatalytic activity towards the redox of bisphenol A. Differential pulse voltammetry was applied to the electrochemical determination of bisphenol A with a wide linear concentration range from 0.2 mu M to 96 mu M and a low limit of detection of 0.17 mu M (S/N = 3). This strategy provides a novel and simple approach for sensitive and selective detection of bisphenol A, holding promising applications in the determination of organic pollution in real water samples.