Membrane modification using zwitterion polymers is an excellent strategy to reduce organic fouling and initial bacterial deposition, and functionalization of the membrane surface with antibacterial material can reduce biofilm formation. In this study, we combined these two approaches to develop an ultrafiltration polyethersulfone (PES) membrane with dual antifouling and antibacterial properties. At the beginning, a zwitterion polyampholyte hydrogel was UV grafted onto PES membrane surface (p-PES membrane). Then, the hydrogel was loaded with graphene oxide (GO) nanosheets using vacuum filtration strategy (GO-p-PES membrane). Raman spectroscopy and scanning electron microscopy (SEM) confirmed the successful incorporation of the GO nanosheets into the zwitterion hydrogel, and contact angle measurements indicated that the membrane hydrophilicity was enhanced. Static adsorption and dynamic filtration experiments demonstrated that the p-PES and GO-p-PES membranes exhibited similar organic fouling propensity. Moreover, the loading of GO induced antibacterial property for the membrane as evidenced by contact killing and antibiofouling filtration experiments. Leaching of GO was very low, with over 98% of the GO remaining on the membrane surface after 7 days. Our findings highlight the potential of this GO-functionalized polyampholyte hydrogel for long-term wastewater treatment.