A novel one-pot strategy was carried out to synthesize Au-Fe3O4-GO nanocomposites, which could be applied for enhanced electrochemical sensing of hydrazine. The synthesis method combined redox reaction and co-precipitation reaction. Fe(II) ion was simultaneously used as a reductant and co-precipitation agent. Then, the nanocomposites were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), energy-dispersive spectroscopy (EDS). In addition, the performance of hydrazine sensor was investigated. The results indicated that Au-Fe3O4-GO/GCE showed superior catalytic capability for hydrazine oxidation. The linear range was approximately from 3.8 mu M to 1.4 mM with a high sensitivity of 308.1 mu A mM(-1) cm(-2) and a low detection limit of 0.9 mu M (S/N = 3). Moreover, it was applied to testing hydrazine in tap water with a desirable recovery among 97.8% to 101.0%. Therefore, the facile preparation method made the sensing material promising for the construction of other electrochemical sensors and bioanalysis. (C) 2018 The Electrochemical Society.