In this study, Chiral Metamaterial Sensor (CMS), which is designed by utilizing a Split Ring Resonators (SRR) has been employed to reveal the two different types of sensor applications by experimentally and numerically. These applications include purity demonstration of methanol, ethanol and isopropyl alcohol (IPA) and fuel adulteration applications for petrol. All of the validations have been realized by measuring the complex permittivity values and reflection coefficients by using a vector network analyzer and a sample holder. For simulation, FIT based simulation program is employed. Both numerical and experimental study results are complying with each other. Sample holder which is developed in this study for microfluidic measurements can easily be tuned for other microfluidic sensor applications thanks to its unique design. Having 280MHz bandwidth with -40dB signal level indicates that the proposed structure is highly sensitive and gives a linear output according to purity and adulteration applications. Whereas, there are many metamaterial researches on purity applications in literature, this is the first study that investigates the purity of methanol, ethanol, isopropyl alcohol (IPA) and fuel adulteration of petrol by using a novel chiral metamaterial sensor designed specifically for the proposed applications. (C) 2018 The Electrochemical Society.