Herein, a novel host-guest recognition among tryptophan (Trp)-linkedDNA, methyl viologen (MV2+) and cucurbit[ 8] uril (CB[8]) was introduced to construct a biosensor for the sensitive assay of oral cancer gene based on nicking enzyme signaling amplification (NESA) and Pt & Pd-MoS2 nanomaterial for the first time. A bioconjugates containing alkaline phosphatase (ALP) and complementary strand (S1) was designed as signal probe to catalyze the 1-naphthyl phosphate monosodium salt monohydrate (NPP) to in situ generate massive electroactive substance 1-naphthol, resulting in the generation of significant peak current with use of Pt & Pd-MoS2 nanomaterial as a catalyst. With target recycling amplification and highly efficient catalysis of ALP, the proposed biosensor for target DNA exhibited a satisfactory linear range from 10 fM to 10 nM. Importantly, the admirable performance of the proposed method shows a potential in biomedical research and clinical diagnosis. (c) 2018 The Electrochemical Society.