An ultrasensitive voltammetric sensor for the resolution of tert-butyl hydroquinone (TBHQ) and propyl gallate (PG) at submicromolar levels has been developed. Acetylene black modified gold electrode (AB/GE) has been recognized to be a simple yet highly sensitive sensing platform enabling the individual determination of these antioxidants within a wide range of concentration from 1.0 x 10(-4) M to 3.0 x 10(-7) M and 1.0 x 10(-4) M to 7.0 x 10(-8) M with detection limits 2.8 x 10(-8) M and 4.3 x 10(-8) M for TBHQ and PG respectively. Moreover, highly resolved peaks for the oxidation of TBHQ and PG with a separation of 206 mV have been observed on AB/GE which in turn facilitated their simultaneous determination. The potential of the developed sensor toward the quantification of TBHQ and PG in fats and oils has proved its efficacy as a promising tool for food quality control. (C) 2018 The Electrochemical Society.