The present research work is an in vivo study that aimed to evaluate the potential role of bioglass-chitosan (BG-CS) and bioglass-chitosan-20%ciprofloxacin (BG-CS-20Cip) in antioxidant profile and osteointegration. These scaffolds were implanted in the defect bone of femoral condyles in ovariectomized rats. The treatment with BG-CS-20Cip has shown a significantly higher stress proteins concentration in comparison with that implanted with BG-CS group. The thiol and vitamin C in BG-CS-20Cip group were significantly enhanced when compared with those in BG-CS group. The histological and physicochemical analyses highlight the BG-CS implications in the bone construction. This property was found to decrease with the presence of ciprofloxacin that caused the delay of this phenomenon. ICP-OES has revealed that the introduction of this antibiotic to the composite led to decrease bone mineralization by evaluating Ca/P ratio. The SEM results have confirmed a progressive degradation of BG-CS and BG-CS-20Cip. However, such bioresorbability and bioactivity of BG-CS was proven to be faster than those of BG-CS-20Cip. Therefore, the incorporation of ciprofloxacin in BG-CS was characterized by a delaying effect of composite dissolution and the formation of apatitic phase. The development of BG-CS as a therapeutic biomaterial protector against oxidative stress is likely to make an effective choice for the application in tissue engineering.