Zinc (Zn) is a necessary microelement for microorganisms to perform various vital functions, which can influence the diversity of microorganisms and get them involved in pollutant degradation in biological wastewater treatment plant. In this study, a novel biocarrier containing different weight fraction (wt%) of zinc nanoparticles (Zn NPs) (0, 10, 15, 20 and 30 wt%) were developed by mixing and melt compounding technology to deal with textile wastewater (TW). The moving bed biofilm reactor (MBBR) filled with biocarriers containing 20 wt% Zn NPs, with zinc ion release profile of 0.4 mg/L had the best performance. It could shorten the start-up time to 11 d, and had higher attached biomass (2460 mg/L) and biofilm dehydrogenase activity (nearly 350 g TPF/g). As a result, the removal efficiencies of COD and NH3-N were higher than reactors filled with other biocarriers. It indicate that the biocarriers containing Zn NPs is expected to increase the metabolic activity of microorganisms and to improve pollutant removal efficiency in the bioflim reactor for TW treatment.