자소엽 추출물의 항균 및 항산화 효과와 성분분석

정효진; 현송화; 송바름; 이상래; 이윤주; 박수남; Hyo Jin Jeong; Song Hua Xuan; Ba Reum Song; Sang Lae Lee; Yun Ju Lee; Soo Nam Park

Applied Chemistry for Engineering, Vol.29, No.6, 716-725, December, 2018

DOI10.14478/ace.2018.1077 Export Citation

자소엽 추출물의 항균 및 항산화 효과와 성분분석

Antimicrobial and Antioxidant Activities of Perilla frutescens var. acuta Extract and Its Fraction and Their Component Analyses

정효진, 현송화, 송바름, 이상래, 이윤주, 박수남 ^†

서울과학기술대학교 정밀화학과

Hyo Jin Jeong, Song Hua Xuan, Ba Reum Song, Sang Lae Lee, Yun Ju Lee, and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology, 232, Gongneung-ro, Nowon-gu, Seoul 01811, Korea

E-mail:

초록

본 연구에서는 자소엽의 50% 에탄올 추출물 및 에틸아세테이트 분획을 제조하여 항균 및 항산화 활성을 평가하고 성분 분석을 진행하였다. 피부 상재균에 대한 항균 활성을 측정한 결과, S. aureus 및 P. aeruginosa에 대해서 에틸아세 테이트 분획의 최소저해농도(minimum inhibitory concentration, MIC) 값은 모두 78 μg/mL로 나타나 항균 활성이 매우 우수함을 확인하였다. 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거 활성(FSC50)과 Fe3+-EDTA/H2O2계에서의 활성산소 소거 활성(OSC50) 측정 결과 50% 에탄올 추출물 보다 에틸아세테이트 분획에서 높은 활성을 보였고 각각 25.90, 1.40 μg/mL로 나타났다. HaCaT 세포에서 세포 내 ROS 억제 효능은 최고 농도인 32 μg/mL에서 50% 에탄올 추출물과 에틸아세테이트 분획 각각 PC군 대비 28.6, 40.7%의 감소율을 보였고 UVB에 대한 세포보호효과는 에틸아세테이트분획의 2.0-16.0 μg/mL 농도에서 농도 의존적으로 나타났다. 자소엽 추출물의 에틸아세테이트 분획을 이용한 성분 분석 결과 rosmarinic acid, luteolin, apigenin, caffeic acid 및 ethyl caffeate가 있음을 확인하였다. 이상의 결과들은 자소엽추출물 및 분획이 항균 및 항산화 효과를 가짐을 보여주며 외부 환경으로부터 피부를 보호할 수 있는 천연 소재로써 화장품 분야에서 응용 가능성이 있음을 시사한다.

In this study, antimicrobial and antioxidative activities of Perilla frutescens var. acuta were investigated with 50% ethanol and the ethyl acetate fraction and also the components were analyzed. The minimum inhibitory concentration (MIC) of the ethyl acetate fraction for both Staphylococcus aureus and Pseudomonas aeruginosa were 78 μg/mL, indicating high antimicrobial effects. The free radical scavenging activity (FSC50) and the reactive oxygen species (ROS) scavenging activity (OSC50) in Fe3+-EDTA/H2O2 system values of the ethyl acetate fraction were 25.90 μg/mL and 1.40 μg/mL, respectively. After the cell damage induced by 400 mJ/cm2 UVB irradiation, the cytoprotective effect of the ethyl acetate fraction of P. frutescens var. acuta showed the concentration dependent manner ranging from 2.0 to 16.0 μg/mL. The intracellular ROS inhibitory activity in HaCaT cells decreased to 28.6% and 40.7% for the 50% ethanol extract and ethyl acetate fraction, respectively at the concentration of 32 μg/mL. Components of rosmarinic acid, luteolin, apigenin, caffeic acid and ethyl caffeate were identified in the ethyl acetate fraction. These results suggest that the extract and fraction of P. frutescens var. acuta may be applied to the field of cosmetics as a natural material that protects the skin from an external environment by having antimicrobial and antioxidative activities.

Keywords:Perilla frutescens;antioxidative activity;reactive oxygen species;antimicrobial effect;flavonoid

[References]

Grice EA, Segre JA, Nat. Rev. Microbiol., 9, 244 (2011)
Yi MR, Jeon AL, Kang CH, Bu HJ, J. Korean Oil. Chem. Soc., 33, 717 (2016)
Kim JH, Kim MJ, Choi SK, Bae SH, An SK, Yoon YM, J. Soc. Cosmet. Sci. Korea, 37, 303 (2011)
Choi EY, Korean J. Aesthet. Cosmetol., 13, 213 (2015)
Yeo YH, Park CH, Liriope platyphylla, Korean Soc. Biotechnol. Bioeng. J., 29, 372 (2014).
Pillai S, Oresajo C, Hayward J, Int. J. Cosmet. Sci., 27, 17 (2005)
Kammeyer A, Luiten RM, Ageing Res. Rev., 21, 16 (2015)
Wlaschek M, Tantcheva-Poor I, Naderi L, Ma WJ, Schneider A, Razi-Wolf Z, Schuller J, Scharffetter-Kochanek K, J. Photochem. Photobiol., 63, 41 (2001)
Song WY, Choi JH, J. Life Sci., 27, 180 (2017)
Soto ML, Parada M, Falque E, Dominguez H, Cosmetics, 5, 13 (2018)
Namngam C, Pinsirodom P, Trop. J. Pharm. Res., 16, 9 (2017)
Kim EC, Ahn SY, Hong ES, Li GH, Kim EK, Row KH, J. Korean Ind. Eng. Chem., 16, 348 (2005)
Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridha JB, Free Rad. Res., 22, 375 (1995)
Griffiths K, Aggarwal BB, Singh RB, Buttar HS, Wilson D, Meester FD, Diseases, 4, 28 (2016)
Kim MH, Kang WW, Lee NH, Kwoen DJ, Choi UK, J. Korean Soc. Appl. Biol. Chem., 50, 327 (2007)
Park DY, Lee KY, J. Korea Acad. Ind. Coop. Soc., 18, 513 (2017)
You JS, Kim SY, Kim SH, Shin TY, Korean J. Pharmacogn., 43, 163 (2012)
Bae JS, Han MR, Shin HS, Kim MK, Shin CY, Lee DH, Chung JH, J. Ethnopharmacol., 195, 334 (2017)
Kim MH, Lee NH, Lee MH, Kwon DJ, Choi UK, Korean J. Food. Cult., 22, 266 (2007)
Nakamura Y, Ohto Y, Murakami A, Ohigashi H, J. Agric. Food. Chem., 46, 4545 (1998)
Kim DH, Kim YC, Choi UK, Int. J. Mol. Sci., 12(4), 2395 (2011)
Kim M, Park YG, Lee HJ, Lim SJ, Nho CW, J. Agric. Food Chem., 63, 5428 (2015)
Alves RC, Costa ASG, Jerez M, Casal S, Sineiro J, Nunez MJ, Oliveira B, J. Agric. Food. Chem., 58, 12221 (2010)
Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, Cherif JK, Ayadi MT, J. Appl. Pharm. Sci., 5, 52 (2014)
Park SH, Seong JS, Lee KS, Park YM, Xuan SH, Cha MY, Kang HC, Park SN, J. Soc. Cosmet. Sci. Korea, 43, 255 (2017)
Stojiljkovic D, Pablobic D, Arsic I, Acta Fac. Med. Naissensis, 31, 207 (2014)
Amaro-Ortiz A, Yan B, D’Orazio JA, Molecules, 19, 6202 (2015)
Yu HA, Kim CD, Asian. J. Beauty Cosmetol., 15, 132 (2017)
Ha MJ, You SH, Asian. J. Beauty Cosmetol., 14, 463 (2016)
Meng L, Lozano YF, Gaydou EM, Li B, Molecules, 14, 133 (2009)
Kang NS, Lee JH, Food Chem., 124, 556 (2011)
Chiang YM, Lo CP, Chen YP, Wang SY, Yang NS, Kuo YH, Shyur LF, Br. J. Pharmacol., 146, 352 (2005)

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[2] Yi MR, Jeon AL, Kang CH, Bu HJ, J. Korean Oil. Chem. Soc., 33, 717 (2016)

[3] Kim JH, Kim MJ, Choi SK, Bae SH, An SK, Yoon YM, J. Soc. Cosmet. Sci. Korea, 37, 303 (2011)

[4] Choi EY, Korean J. Aesthet. Cosmetol., 13, 213 (2015)

[5] Yeo YH, Park CH, Liriope platyphylla, Korean Soc. Biotechnol. Bioeng. J., 29, 372 (2014).

[6] Pillai S, Oresajo C, Hayward J, Int. J. Cosmet. Sci., 27, 17 (2005)

[7] Kammeyer A, Luiten RM, Ageing Res. Rev., 21, 16 (2015)

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[9] Song WY, Choi JH, J. Life Sci., 27, 180 (2017)

[10] Soto ML, Parada M, Falque E, Dominguez H, Cosmetics, 5, 13 (2018)

[11] Namngam C, Pinsirodom P, Trop. J. Pharm. Res., 16, 9 (2017)

[12] Kim EC, Ahn SY, Hong ES, Li GH, Kim EK, Row KH, J. Korean Ind. Eng. Chem., 16, 348 (2005)

[13] Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridha JB, Free Rad. Res., 22, 375 (1995)

[14] Griffiths K, Aggarwal BB, Singh RB, Buttar HS, Wilson D, Meester FD, Diseases, 4, 28 (2016)

[15] Kim MH, Kang WW, Lee NH, Kwoen DJ, Choi UK, J. Korean Soc. Appl. Biol. Chem., 50, 327 (2007)

[16] Park DY, Lee KY, J. Korea Acad. Ind. Coop. Soc., 18, 513 (2017)

[17] You JS, Kim SY, Kim SH, Shin TY, Korean J. Pharmacogn., 43, 163 (2012)

[18] Bae JS, Han MR, Shin HS, Kim MK, Shin CY, Lee DH, Chung JH, J. Ethnopharmacol., 195, 334 (2017)

[19] Kim MH, Lee NH, Lee MH, Kwon DJ, Choi UK, Korean J. Food. Cult., 22, 266 (2007)

[20] Nakamura Y, Ohto Y, Murakami A, Ohigashi H, J. Agric. Food. Chem., 46, 4545 (1998)

[21] Kim DH, Kim YC, Choi UK, Int. J. Mol. Sci., 12(4), 2395 (2011)

[22] Kim M, Park YG, Lee HJ, Lim SJ, Nho CW, J. Agric. Food Chem., 63, 5428 (2015)

[23] Alves RC, Costa ASG, Jerez M, Casal S, Sineiro J, Nunez MJ, Oliveira B, J. Agric. Food. Chem., 58, 12221 (2010)

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[25] Park SH, Seong JS, Lee KS, Park YM, Xuan SH, Cha MY, Kang HC, Park SN, J. Soc. Cosmet. Sci. Korea, 43, 255 (2017)

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[29] Ha MJ, You SH, Asian. J. Beauty Cosmetol., 14, 463 (2016)

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