화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.72, 457-473, April, 2019
DOI10.1016/j.jiec.2019.01.002  Export Citation
phytosynthesis of zinc oxide nanoparticles and its antibacterial, antiquorum sensing, antimotility, and antioxidant capacities against multidrug resistant bacteria
Mehran Alavi, Naser Karimi†, and Iraj Salimikia1, †
  • Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
E-mail:,
It was used green biosynthesized zinc oxide (ZnO) NPs (average diameter of 50-60 nm) with abilities of antibacterial against sensetive and MDR bacteria including Escherichia coli ATCC 25922, Serratia marcescens ATCC13880, Pseudomonas aeruginosa ATCC 27853 (gram negative), and Staphylococcus aureus ATCC 43300 and Staphylococcus epidermidis ATCC 12258 (gram positive). High reduction in biofilm formation was 63.43% (0.562 ± 0.015) and 62.88% (0.582 ±0.025) at 100 μg/mL concentration against control for S. epidermidis and P. aeruginosa respectively. In conclusion, this investigation illustrated simple green synthesis of ZnO NPs with suitable advantages of antioxidant, antibacterial, anti biofilm, anti-QS activities, and also anti-swarming motility against MDR bacteria.
Keywords:Biosynthesized ZnO NPs;MDR bacteria;Antibacterial;Anti-biofilm;Anti-quorum sensing;Antioxidant
  1. Blair JMA, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJV, Nat. Rev. Microbiol., 13, 42 (2014)
  2. Lipsky BA, Aragon-Sanchez J, Diggle M, Embil J, Kono S, Lavery L, Senneville E, Urbancic-Rovan V, Van Asten S, Peters EJG, Diabetes Metab. Res. Rev., 32, 45 (2016)
  3. Pelgrift RY, Friedman AJ, Adv. Drug Deliv. Rev., 65, 1803 (2013)
  4. Rossolini GM, Arena F, Pecile P, Pollini S, Curr. Opin. Pharmacol., 18, 56 (2014)
  5. Ciofu O, Tolker-Nielsen T, Jensen PO, Wang H, Høiby N, Adv. Drug Deliv. Rev., 85, 7 (2015)
  6. Lee J, Zhang L, Protein Cell, 6, 26 (2015)
  7. Tayabali AF, Coleman G, Nguyen KC, PLoS One, 10, e01436 (2015)
  8. Curran CS, Bolig T, Torabi-Parizi P, Am. J. Respir. Crit. Care Med. (2017).
  9. Elkassas D, Arafa A, Nanomed. Nanotechnol. Biol. Med., 13, 1543 (2017)
  10. Alavi M, Karimi N, Artif. Cell Nanomed. Biotechnol., 1 (2017).
  11. Alavi M, Karimi N, Artif. Cells Nanomed. Biotechnol., 1 (2018).
  12. Kaviyarasu K, Geetha N, Kanimozhi K, Magdalane CM, Sivaranjani S, Ayeshamariam A, Kennedy J, Maaza M, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 74, 325 (2017)
  13. Taran M, Rad M, Alavi M, Bioimpacts, 8, 65 (2018)
  14. Orou SFC, Hang KJ, Thien MT, Lee YY, Foh LC, Diem NDN, Hee GB, Yong PS, Fen PY, J. Ind. Eng. Chem., 62, 333 (2018)
  15. Shafie ZMHM, Ahmad AL, J. Ind. Eng. Chem., 62, 273 (2018)
  16. Tan L, Wu W, Yin Q, Ke X, Qiao R, Tong G, Zhu L, J. Ind. Eng. Chem., 52, 349 (2017)
  17. Li C, Zang Z, Han C, Hu Z, Tang X, Du J, Leng Y, Sun K, Nano Energy, 40, 195 (2017)
  18. Li CL, Han C, Zhang YB, Zang ZG, Wang M, Tang XS, Du JH, Sol. Energy Mater. Sol. Cells, 172, 341 (2017)
  19. Zang Z, Appl. Phys. Lett., 112, 042106 (2018)
  20. Lup ANK, Abnisa F, Daud WMAW, Aroua MK, J. Ind. Eng. Chem., 56, 1 (2017)
  21. Chhowalla M, Shin HS, Eda G, Li LJ, Loh KP, Zhang H, Nat. Chem., 5, 263 (2013)
  22. Zang Z, Tang X, J. Alloy. Compd., 619, 98 (2015)
  23. Khan MF, Ansari AH, Hameedullah M, Ahmad E, Husain FM, Zia Q, Baig U, et al., Sci. Rep., 6, 27689 (2016)
  24. Singh P, Kim YJ, Wang C, Mathiyalagan R, Yang DC, Artif. Cells Nanomed. Biotechnol., 44, 1150 (2016)
  25. Saravanakumar A, Peng MM, Ganesh M, Jayaprakash J, Mohankumar M, Jang HT, Artif. Cells Nanomed. Biotechnol., 45, 1165 (2017)
  26. Sangeetha G, Rajeshwari S, Venckatesh R, Mater. Res. Bull., 46(12), 2560 (2011)
  27. Gouveia S, Castilho PC, Food Res. Int., 44, 1620 (2011)
  28. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YJ, J. Food Drug Anal., 22, 296 (2014)
  29. Taleb H, Maddocks SE, Morris RK, Kanekanian AD, Front. Microbiol., 7, 198 (2016)
  30. Sun B, Ricardo-da-Silva JM, Spranger I, J. Agric. Food. Chem., 46, 4267 (1998)
  31. Baskaran X, Vigila AVG, Parimelazhagan T, Muralidhara-Rao D, Zhang S, Int. J. Nanomed., 11, 5789 (2016)
  32. Iqbal J, Siddiqui R, Kazmi SU, Khan NA, Biomed Res. Int., 2013, 927323 (2013)
  33. Odabasi Z, Paetznick V, Goldstein BP, Rex JH, Ostrosky-Zeichner L, Antimicrob. Agents Chemother., 47, 3018 (2003)
  34. Elumalai K, Velmurugan S, Appl. Surf. Sci., 345, 329 (2015)
  35. Kelley KD, Olive LQ, Hadziselimovic A, Sanders CR, Biochemistry, 49, 5405 (2010)
  36. Zarei M, Jamnejad A, Khajehali E, Jundishapur J. Microbiol., 7, e8720 (2014)
  37. Schacht VJ, Neumann LV, Sandhi SK, Chen L, Henning T, Klar PJ, Theophel K, Schnell S, Bunge M, J. Appl. Microbiol., 114(1), 25 (2013)
  38. Arakha M, Saleem M, Mallick BC, Jha S, Sci. Rep., 5 (2015)
  39. Kong C, Chee CF, Richter K, Thomas N, Abd. Rahman N, Nathan S, Sci. Rep., 8, 2758 (2018)
  40. Hoffman LR, D'Argenio DA, MacCoss MJ, Zhang Z, Nature, 436, 1171 (2005)
  41. O'May C, Tufenkji N, Appl. Environ. Microbiol., 77, 3061 (2011)
  42. Vinckx T, Wei Q, Matthijs S, Cornelis P, Microbiology, 156, 678 (2010)
  43. Carson CF, Hammer KA, Riley TV, Clin. Microbiol. Rev, 19, 50 (2006)
  44. Niu YH, Yeung LK, Crooks RM, J. Am. Chem. Soc., 123(28), 6840 (2001)
  45. Chen XM, Wu GH, Chen JM, Chen X, Xie ZX, Wang XR, J. Am. Chem. Soc., 133(11), 3693 (2011)
  46. Sharma VK, Siskova KM, Zboril R, Gardea-Torresdey JL, Adv. Colloid Interface Sci., 204, 15 (2014)
  47. Phull AR, Abbas Q, Ali A, Raza H, kim SJ, Zia M, Haq IU, Future J. Pharm. Sci., 2, 31 (2016)
  48. Brewer MS, Compr. Rev. Food Sci. Food Saf., 10, 221 (2011)
  49. Mira L, Fernandez MT, Santos M, Rocha R, Florencio MH, Jennings KR, Free Radical Res, 36, 1199 (2002)
  50. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO, Acta Naturae, 6 (2014)
  51. Granger DN, Kvietys PR, Redox Biol., 6, 524 (2015)
  52. Manna AC, Synthesis, Characterization, and Antimicrobial Activity of Zinc Oxide Nanoparticles, pp.151 2012.
  53. Jamdagni P, Khatri P, Rana JS, J. King Saud Univ. - Sci., 30(2), 168 (2018)
  54. Salunke GR, Ghosh S, Kumar RJS, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA, Int. J. Nanomed., 9, 2635 (2014)
  55. Suresh D, Nethravathi PC, Udayabhanu, Rajanaika H, Nagabhushana H, Sharma SC, Mater. Sci. Semicond. Process, 31, 446 (2015)
  56. Elumalai K, Velmurugan S, Ravi S, Kathiravan V, Ashokkumar S, Mater. Sci. Semicond. Process, 34, 365 (2015)
  57. Safarifard V, Morsali A, Coord. Chem. Rev., 292, 1 (2015)
  58. Mathew S, Prasad AK, Benoy T, Rakesh PP, Hari M, Libish TM, Radhakrishnan P, Nampoori VPN, Vallabhan CPG, J. Fluoresc., 22, 1563 (2012)
  59. Baer DR, Gaspar DJ, Nachimuthu P, Techane SD, Castner DG, Anal. Bioanal. Chem., 396, 983 (2010)
  60. Lin PC, Lin S, Wang PC, Sridhar R, Biotechnol. Adv., 32, 711 (2014)
  61. Elumalai K, Velmurugan S, Ravi S, Kathiravan V, Ashokkumar S, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 143, 158 (2015)
  62. Wulandari L, Retnaningtyas Y, Nuri, Lukman H, J. Anal. Methods Chem., 2016, 469680 (2016)
  63. Cheviron P, Gouanve F, Espuche E, Carbohydr. Polym., 108, 291 (2014)
  64. Santhoshkumar T, Rahuman AA, Jayaseelan C, Rajakumar G, Marimuthu S, Kirthi AV, Velayutham K, Thomas J, Venkatesan J, Kim SK, Asian Pac. J. Trop. Med., 7, 968 (2014)
  65. Kim TY, Cha SH, Cho S, Park Y, Arch. Pharmacal Res., 39, 465 (2016)
  66. Nagarajan S, Kuppusamy KA, J. Nanobiotechnol., 11, 39 (2013)
  67. Bertoni G, Fabbri F, Villani M, Lazzarini L, Turner S, Van Tendeloo G, Calestani D, Gradecak S, Zappettini A, Salviati G, Sci. Rep., 6, 19168 (2016)
  68. Geetha MS, Nagabhushana H, Shivananjaiah HN, J. Sci.: Adv. Mater. Devices, 1, 301 (2016)
  69. Elumalai K, Velmurugan S, Appl. Surf. Sci., 345, 329 (2015)
  70. Bala N, Saha S, Chakraborty M, Maiti M, Das S, Basu R, Nandy P, RSC Adv., 5, 4993 (2015)
  71. Arakha M, Pal S, Samantarrai D, Panigrahi TK, Mallick BC, Pramanik K, Mallick B, Jha S, Sci. Rep., 5, 14813 (2015)
  72. Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A, Int. J. Nanomed., 7, 6003 (2012)
  73. Silhavy TJ, Kahne D, Walker S, Cold Spring Harbor Perspect. Biol., 2, a00041 (2010)
  74. Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, Hasan H, Mohamad D, Nano-Micro Lett., 7, 219 (2015)
  75. Dobrucka R, Długaszewska J, Saudi J. Biolo. Sci., 23, 517 (2016)
  76. Cavassin ED, de Figueiredo LFP, Otoch JP, Seckler MM, de Oliveira RA, Franco FF, Marangoni VS, Zucolotto V, Levin ASS, Costa SF, J. Nanobiotechnol., 13, 64 (2015)
  77. Ocampo PS, Lazar V, Papp B, Arnoldini M, Abel zur Wiesch P, Busa-Fekete R, Fekete G, Pal C, Ackermann M, Bonhoeffer S, Antimicrob. Agents Chemother., 58, 4573 (2014)
  78. Lu HD, Yang SS, Wilson BK, McManus SA, Prud’homme CCVHHRK, Appl. Nanosci., 7, 83 (2017)
  79. Scott JR, Barnett TC, Annu. Rev. Microbiol., 60, 397 (2006)
  80. Ashkarran AA, Ghavami M, Aghaverdi H, Stroeve P, Mahmoudi M, Chem. Res. Toxicol., 25, 1231 (2012)
  81. Arakha M, Saleem M, Mallick BC, Jha S, Sci. Rep., 5, 9578 (2015)
  82. He Y, Ingudam S, Reed S, Gehring A, Strobaugh TP, Irwin P, J. Nanobiotechnol., 14, 54 (2016)
  83. Salem W, Leitner DR, Zingl FG, Schratter G, Prassl R, Goessler W, Reidl J, Schild S, Int. J. Med. Microbiol., 305, 85 (2015)
  84. Madhumitha G, Elango G, Roopan SM, Appl. Microbiol. Biotechnol., 100(2), 571 (2016)
  85. Lee JH, Kim YG, Cho MH, Lee J, Microbiol. Res., 169, 888 (2014)
  86. Applerot G, Lellouche J, Perkas N, Nitzan Y, Gedanken A, Banin E, RSC Adv., 2, 2314 (2012)
  87. LewisOscar F, MubarakAli D, Nithya C, Priyanka R, Gopinath V, Alharbi NS, Thajuddin N, Biofouling, 31, 379 (2015)
  88. Vijayakumar S, Vinoj G, Malaikozhundan B, Shanthi S, Vaseeharan B, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 137, 886 (2015)
  89. Gold K, Slay B, Knackstedt M, Gaharwar AK, Adv. Ther., 1700033 (2018).
  90. Joe A, Park SH, Shim KD, Kim DJ, Jhee KH, Lee HW, Heo CH, Kim HM, Jang ES, J. Ind. Eng. Chem., 45, 430 (2017)
  91. Salunke GR, Ghosh S, Kumar RJS, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA, Int J. Nanomed., 9, 2635 (2014)
  92. Flemming HC, Wingender J, Nat. Rev. Microbiol., 8, 623 (2010)
  93. Kohler T, Curty LK, Barja F, van Delden C, Pechere JC, J. Bacteriol., 182, 5990 (2000)
  94. Singh BR, Singh BN, Singh A, Khan W, Naqvi AH, Singh HB, Sci. Rep., 5, 13719 (2015)
  95. Rajkumari J, Busi S, Vasu AC, Reddy P, Microb. Pathog., 107, 261 (2017)
  96. Nafee N, Husari A, Maurer CK, Lu C, de Rossi C, Steinbach A, Hartmann RW, Lehr CM, Schneider M, J. Control. Release, 192, 131 (2014)
  97. Muller M, Merrett ND, Antimicrob. Agents Chemother., 58, 5492 (2014)