Layered Ti3C2Tx powders have been experimentally proved to hold high adsorption activity, while it is useless to be an efficient demulsifier. Pure chitosan powders with excellent adsorption are easy to dissolve in acidic solution, so it is difficult to break of acid oil-in-water emulsions. In order to expand the application fields in breaking up the emulsified oily waste acid water, chitosan loaded Ti3C2Tx powders have been successfully fabricated by simple means, which can be used to strong acidic emulsions and improve the use ratio of acid water. Chitosan loaded Ti3C2Tx powders possess higher specific surface area than that of Ti3C2Tx powders, so these powders possess more reactive activation. In addition, chitosan loaded Ti3C2Tx powders would be applied to high-temperature condition, which possess extensive temperature limit (< 200 degrees C). In the work, the demulsification efficiency can be up to about 67% with the increasing dosage of chitosan loaded Ti3C2Tx powders, while the layered structure of this demulsifier keeping original after demulsification.