In this study, two cycles of mixotrophic and one cycle of heterotrophic cultivation of Scenedesmus quadricauda (freshwater) and Tetraselmis suecica (marine water) microalgae in dairy wastewater (DWW) were investigated. Dry weights of S. quadricauda and T. suecica were found to be 0.43 and 0.58 g/L after the first cycle and 0.36, and 0.65 g/L after the second cycle of mixotrophic cultivation, respectively. Chlorophyll a content of both microalgae in the first cycle was significantly higher than the second cycle. S. quadricauda removed 92.15% of total nitrogen, 100% of phosphate, 100% of sulfate and 76.77% of total organic carbon, after two cycles of cultivation. The dominant fatty acids during the first and second cycle of S. quadricauda and T. suecica cultivation were C18:1 and C18:3n-3, respectively. The results suggest that by reusing DWW in two consecutive cycles of microalgal cultivation, higher pollutants removal efficiency and microalgal biomass production can be achieved.