Ni-based catalyst is prone to the deactivation by coke deposition during the catalytic steam reforming of bio-oil. In order to optimize catalytic activity of the Ni-based catalyst, a two-stage catalytic reforming scheme was proposed whereby a low-temperature (350-600 degrees C) pre-reforming process with a less expensive catalyst was introduced prior to the Ni-based catalyst. To study this scheme, the reforming experiments with different combinations of Fe/bio-char (in the first stage) and Ni-Ca/gamma-Al2O3 (in the second stage) were conducted. In addition to the quantification of product yields, the tar and gas were characterized with an ultraviolet (UV) fluorescence spectroscopy, a gas chromatography/mass spectrometer (GC/MS) and a gas chromatography (GC). The results indicate that significant proportions of naphthalenes and polycyclic aromatics in the intermediate volatiles turned into furans, phenols and non-aromatics, which are easier to be reformed on Ni-based catalysts, via the Fe/bio-char pre-reforming, resulting in the increase of the final H-2 and CO yields of the overall process. At high pre-reforming temperatures (550-600 degrees C), the coke formation on Ni-based catalyst was significantly inhibited due to the Fe/bio-char pre-reforming. In addition, the results indicate that the spent Ni-based catalyst had more free NiO and NiAl2O4 spinel than the fresh one, suggesting that the increase in the catalytic activity of Ni-based catalyst during the reforming process was promoted by Fe/bio-char pre-reforming.