A novel magnetic Pickering interfacial catalyst (MPIC) was developed and used for highly effective and clean production of biodiesel. The catalyst was synthesized by immobilization of 1,1,3,3-tetramethylguanidine (TMG) onto the shell of Fe3O4@Chloromethyl PS nanohybrids. The resultant Fe3O4@PS-TMG was measured by transmission electron microscope (TEM), dynamic lighting scattering (DLS), Fourier transform infrared spectroscopy (FTIR), elemental analysis (EA), thermogravimetric analysis (TG), contact angles (CA). The results showed that surface property and TMG loading of catalyst can be finely regulated via adjusting the feeding of TMG. Fe3O4@PS-TMG with amphiphilic surface can stabilize soybean oil-in-methanol Pickering emulsion, which would offer large interfacial area and surface enrichment of reactants, leading to a significant enhancement of such liquid-liquid (L-L) biphasic reaction with high mass transfer resistance. Furthermore, the MPIC can be facile separated from the mixture of reaction by magnet, and showed excellent stability over 5 recycles. (C) 2018 Elsevier Inc. All rights reserved.