The merits of microfluidics bring new opportunities for engineering of nanomaterials with well controlled chemical, physical and biological properties for a variety of applications. Herein, using a tworun spiral-shaped microfluidic device, we first develop a facile and straightforward flow synthesis strategy to create two-dimensional mesoporous silica nanosheet (MSN). Such MSN exhibits typical hollow sandwich-like bilayer and unique water ripple-like wrinkle surface, which greatly increase the particulate accessibility for mass transfer. The enhanced on-chip enrichment performance of MSN is further confirmed toward different substrates (dye, protein, drug). These findings not only shed new light on microfluidics-enabled chemicals engineering, but also open up numerous new possibilities by microfluidics in adsorption, separation, and biomedical fields. (C) 2018 Elsevier Inc. All rights reserved.