This paper presents an accessible method to prepare giant lipid vesicles (GLVs) with controllable sizes based on the quick formation of patterned lipid films. Lipid solutions naturally penetrate into arrayed micro-apertures on a modified hydrophilic Polydimethylsiloxane (PDMS) chip, and excess lipid films on the surface are removed by a glass slide. Three main factors, the depth and diameter of the micro-apertures and concentration of the lipid solution, were investigated to obtain an optimal preparation condition. Based on this condition, the formed GLVs have a controllable size and narrow size distribution (the standard deviation < 5 mu m). By controlling the diameter of the micro-aperture and concentration of the lipid solution, GLVs with various sizes (23, 48, 66 and 82 mu m) can be formed. (C) 2018 Elsevier Inc. All rights reserved.