Simultaneous administration of multiple bioactive components might produce synergistic effect and offer multiple health benefits. This provides motivation to develop co-delivery system that can simultaneously encapsulate a plurality of bioactive components. In this study, alpha-tocopherol and resveratrol with different solubility were successfully encapsulated into zein nanoparticles. Encapsulation efficiency of alpha-tocopherol and resveratrol reached 96% and 67%, respectively. Size and zeta-potential of zein particles varied from 80 to 122 nm and +27 to +60 mV, respectively. Resveratrol was encapsulated at the portion between the hydrophobic core and the surface of zein particles. About 47% of alpha-tocopherol and 80% of resveratrol were found in the zein particles when stored at 45 degrees C for up to 84 days. alpha-Tocopherol- and resveratrol-loaded zein particles provided a better protective effect on alpha-tocopherol than did alpha-tocopherol-loaded zein particles and eliminated negative effect of alpha-tocopherol on the stability of resveratrol. These results might be useful for the design of a delivery system for the co-encapsulation of several nutrients with different solubilities.