In the present study, we introduce a direct and sensitive voltammetry approach for the simultaneous determination of aspirin (ASA) and omeprazole (OMZ) for patients suffering from cardiovascular diseases and gastrointestinal disorders. Low dose of ASA can be used for the prevention of cardiovascular diseases which is considered the most severe cause of mortality worldwide. On the other hand, OMZ can reduce the risk of gastric ulcers and gastrointestinal damage associated with ASA. The used electrode was constructed by in situ-modification with multi-walled carbon nanotubes, glutamine, and nano-magnetite in presence of sodium dodecyl sulfate (FeGluCNTCP-SDS). Using this sensor, the limits of detection were 11.9 nmol.L-1 and 3.3 nmol.L-1 for ASA and OMZ, respectively. The proposed sensor was used for the determination of OMZ in presence of dopamine, paracetamol, ascorbic acid, and uric acid without noticeable interference in their binary or ternary mixtures. The proposed surface showed good analytical performance toward OMZ determination and has a promising application for the electrochemical sensing of other drugs of pharmaceutical importance. (C) 2019 The Electrochemical Society.