The heterogeneous structure and dynamic balancing nature of surfactant aggregates make them attractive in developing fluorescent sensors. They can provide a number of advantages, e.g., enhanced fluorescence stability and quantum yield, detection capability in aqueous solutions, and easy operation. Thus, various strategies have been used to construct surfactant aggregate-based fluorescent sensors. Surfactant aggregates play various roles in different strategies and realize multiple sensing behaviors. Many new functions have been discovered for surfactant aggregates in constructing fluorescent sensors. In this feature article, we briefly summarize the development of surfactant aggregate-based fluorescent sensors and their applications in three different types of sensing: selective sensing, multiple analyte sensing, and cross-reactive sensing. For each type of sensing, the design strategies and the roles of surfactant aggregates are particularly introduced. An understanding of these aspects will help to expand the applications of surfactant assemblies in the sensing field.