This study evaluates the simultaneous removal of 2,4-dichlorophenoxyacetic acid acid-2,4-D at 70 mu g L-1 and the viability decreasing of Escherichia coli and Klebsiella pneurnoniae from real groundwater samples containing natural amounts of iron (0.39 mg L-1) and natural pH (7.31) in a coupled prototype system powered by a photovoltaic cell (SPh + LPh + H2O2 + RSF + Cl) consisting of photochemical (using both natural sunlight (SPh) and UV-B + A + Visible lamp (LPh) and a 30-L Compound Parabolic Collector-CPC reactor), rapid sand filtration (RSF) and chlorination (Cl, adding of Ca(OCl)(2) to get residual chlorine ranging between 0.2 and 0.6 mg Cl L-1) to drinking water production. Results showed that simple addition of H2O2 10 mg L-1 and both natural sunlight and artificial light irradiation could enhance several natural photo-induced processes among them photocatalytic and photo-Fenton. These radicals could be responsible for both bacteria inactivation and 2,4-D removal from natural groundwater diminishing the addition of substantial amounts of chemicals into water samples. Coupling of photochemical processes with conventional treatments to drinking water production as RSF and Cl at high solar energy dose (150,000 J m(-2)), led to the efficient removal 2,4-D and bacteria from natural groundwater without a significative trihalomethanes production. These results strongly suggest that coupling of helio-photochemical/H2O2 systems with RSF and chlorination treatments seems to be a promising approach to remove chronic and acute risk from groundwater obtaining drinking water with high chemical and microbiological quality. However, despite these encouraging results, bacteria inactivation underwent a detrimental effect when low solar energy doses (75,000 J m(-2)) were tested.