Hydroxyapatite (HA) thin films with a Ca/P ratio of 1.69 and approximately 500 nm in thickness were deposited by radio frequency magnetron sputtering on Ti-6Al-4 V substrates at 100 degrees C and a working pressure of 0.5 Pa. The obtained coatings were thermally treated at 400 and 700 degrees C in argon atmosphere and characterized in terms of elemental and phase composition, morphology, structure and mechanical properties by energy dispersive x-ray spectroscopy, scanning electron microscopy, atomic force microscopy, micro-Raman spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and nanoindentation. It was found that the thermal treatments increase the crystallinity although induce residual tensile stresses formation in the HA coatings, which are greater at high temperatures. Nevertheless, enhances in the mechanical properties: hardness (H), elastic modulus (E) and adhesion strength for the heat treated at 400 degrees C samples compared to the as-deposited coatings were obtained. Values of 7.45 +/- 0.6 GPa (H), 115 +/- 10 GPa (E) and a critical load of 175 +/- 4 mN were measured for those thermally treated samples at 400 degrees C.