Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells

Jaewon Lee; Jungju Oh; Eun-song Lee; Young-Pil Kim; Minhyung Lee

Journal of Industrial and Engineering Chemistry, Vol.73, 182-191, May, 2019

DOI10.1016/j.jiec.2019.01.023 Export Citation

Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells

Jaewon Lee, Jungju Oh, Eun-song Lee¹, Young-Pil Kim¹, and Minhyung Lee^†

Department of Bioengineering, College of Engineering, Hanyang University, Seoul 04763, Republic of Korea
¹Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea

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Prostate cancer is one of the most frequently diagnosed cancers. In this study, a prostate cancer-specific aptamer, Wy5a, was conjugated to polyethylenimine (PEI) with a polyethylene glycol (PEG) linker for prostate cancer-specific gene delivery. PEI-PEG-AS1411 was synthesized as a non-specific control. PEI-PEG-Wy5a formed complexes with plasmid DNA in a spherical form with a diameter of 70 nm and a positive surface-charge. In transfection assays, PEI-PEG-Wy5a had higher gene delivery efficiency to PC-3 prostate cancer cells than did PEI-PEG or PEI-PEG-AS1411, but not in other types of cells. The results suggest that PEI-PEG-Wy5a may be useful for prostate cancer-specific gene delivery.

Keywords:Aptamer;Prostate cancer;Gene delivery;Polyethylene glycol;Polyethylenimine

[References]

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[Referenced By]

[1] Tai S, Sun Y, Squires JM, Zhang H, Oh WK, Liang CZ, J. Huang, Prostate, 71, 1668 (2011)

[2] Cooperberg MR, Park S, Carroll PR, Oncology, 18, 1239 (2004)

[3] Siegel R, Ma J, Zou Z, Jemal A, Cancer CA, J. Clin., 64, 9 (2014)

[4] Kim E, Jung Y, Choi H, Yang J, Suh JS, Huh YM, Kim K, Haam S, Biomaterials, 31, 4592 (2010)

[5] Gotwals P, Cameron S, Cipolletta D, Cremasco V, Crystal A, Hewes B, Mueller B, Quaratino S, Sabatos-Peyton C, Petruzzelli L, Nat. Rev. Cancer, 17, 286 (2017)

[6] El-Aneed A, J. Control. Release, 94, 1 (2004)

[7] Chae SY, Kim HJ, Lee MS, Jang YL, Lee Y, Lee SH, Lee K, Kim SH, Kim HT, Chi SC, Park TG, Jeong JH, Macromol. Biosci., 11, 1169 (2011)

[8] Jeon P, Choi M, Oh J, Lee M, Macromol. Biosci., 15, 1021 (2015)

[9] Xiao S, Liu Z, Deng R, Li C, Fu S, Chen G, Zhang X, Ke F, Ke S, Yu X, J. Control. Release, 258, 130 (2017)

[10] Kim HA, Park JH, Yi N, Lee M, Mol. Pharm., 11, 938 (2014)

[11] Pack DW, Hoffman AS, Pun S, Stayton PS, Nat. Rev. Drug Discov., 4, 581 (2005)

[12] Mangraviti A, Tzeng SY, Kozielski KL, Wang Y, Jin Y, Gullotti D, Pedone M, Buaron N, Liu A, Wilson DR, A.C.S Nano, 9, 1236 (2015)

[13] Lv H, Zhang S, Wang B, Cui S, Yan J, J. Control. Release, 114, 100 (2006)

[14] Wang Y, Luo Y, Bing T, Chen Z, Lu M, Zhang N, Shangguan D, PLoS. One, 9, e10024 (2014)

[15] Zhou J, Rossi J, Nat. Rev. Drug Discov., 16, 181 (2017)

[16] Chen K, Liu B, Yu B, Zhong W, Lu Y, Zhang J, Liao J, Liu J, Pu Y, Qiu L, Nanomed. Nanobiotech, 9, e1438 (2017)

[17] Li L, Hou J, Liu X, Guo Y, Wu Y, Zhang L, Yang Z, Biomaterials, 35, 3840 (2014)

[18] Li X, Yu Y, Ji Q, Qiu L, Nanomed. Nanotech. Biol. Med., 11, 175 (2015)

[19] Reyes-Reyes E, Salipur FR, Shams M, Forsthefel MK, Bates PJ, Mol. Oncol., 9, 1392 (2015)

[20] Soundararajan S, Chen W, Spicer EK, Courtenay-Luck N, Fernandes DJ, Cancer Res., 68, 2358 (2008)

[21] Maier KE, Levy M, Mol. Ther. Method. Clin. Dev., 3 (2016)

[22] Duan M, Long Y, Yang C, Wu X, Sun Y, Li J, Hu X, Lin W, Han D, Zhao Y, Oncotarget, 7, 36436 (2016)

[23] Guan XW, Guo ZP, Wang TH, Lin L, Chen J, Tian HY, Chen XS, Biomacromolecules, 18(4), 1342 (2017)

[24] Luong D, Kesharwani P, Deshmukh R, Amin MCIM, Gupta U, Greish K, Iyer AK, Acta Biomater., 43, 14 (2016)

[25] Wang H, Zhao X, Guo C, Ren D, Zhao Y, Xiao W, Jiao W, PLoS One, 10, e01391 (2015)

[26] Lemkine GF, Goula D, Becker N, Paleari L, Levi G, Demeneix BA, J. Drug Target., 7, 305 (1999)

[27] Godbey W, Wu KK, Mikos AG, J. Control. Release, 60, 149 (1999)

[28] Park TG, Jeong JH, Kim SW, Adv. Drug Deliv. Rev., 58, 467 (2006)

[29] Kim YH, Park JH, Lee M, Kim YH, Park TG, Kim SW, J. Control. Release, 103, 209 (2005)

[30] Taranejoo S, Liu J, Verma P, Hourigan K, J. Appl. Pol. Sci., 132 (2015).

[31] Fischer D, Bieber T, Li Y, Elsasser HP, Kissel T, Pharm. Res., 16, 1273 (1999)