Nanosized and tunable design of biosilica particles using novel silica-forming peptide-modified chimeric ferritin templates

Thi Khoa My Nguyen; Mi Ran Ki; Chang Soo Lee; Seung Pil Pack

Journal of Industrial and Engineering Chemistry, Vol.73, 198-204, May, 2019

DOI10.1016/j.jiec.2019.01.025 Export Citation

Nanosized and tunable design of biosilica particles using novel silica-forming peptide-modified chimeric ferritin templates

Thi Khoa My Nguyen, Mi Ran Ki, Chang Soo Lee^{1, †}, and Seung Pil Pack^†

Department of Biotechnology and Bioinformatics, Korea University, 2511 Sejong-Ro, Sejong 30019, Korea
¹Department of Chemical Engineering, Chungnam National University, 99 Daehak-Ro, Yuseong, Daejeon 34134, Korea

E-mail:,

Biosilica materials can be generated by biomolecules under physiological or mild/ambient conditions. However, nanosized and tunable design method of biosilica particles has not yet been set up. Here, Kps, a silica forming peptide (KPSHHHHHTGAN) was introduced to N-terminus of ferritin via protein fusion method to generate new Kps-modified ferritin (Kps-Fn) for reliable formation of biosilica particles. Then, novel chimeric Kps-Fn was designed for controllable generation of biosilica nanoparticles (NPs). By changing the ratios of Kps-Fn and Fn subunits in the chimeric Kps-Fn templates, desired size of biosilica NPs (100?500 nm) can be achieved. The low surface density of Kps on the chimeric template could lead to the small-sized biosilica NPs with the increased surface area/amount. The biosilica NPs developed here showed better DNA adsorption/elution performance than the commercially available silica NPs. This work is a new way to generate desirable biosilica NPs, which can be applied for the design of various biohybrid nanomaterials.

Keywords:Silica-forming peptide;Ferritin;Biosilica nanoparticle;Biosilicification

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[Referenced By]

[1] Jo BH, Seo JH, Yang YJ, Baek K, Choi YS, Pack SP, Oh SH, Cha HJ, ACS Catal., 4, 4332 (2014)

[2] Ki MR, Yeo KB, Pack SP, Bioprocess Biosyst. Eng., 36, 643 (2013)

[3] Min KH, Son RG, Ki MR, Choi YS, Pack SP, Chemosphere, 143, 128 (2016)

[4] Park KS, Ki MR, Yeo KS, Choi JH, Pack SP, Biochem. Eng. J., 128, 112 (2017)

[5] Ryu YH, Yeo KB, Ki MR, Kim YJ, Pack SP, Biochem. Eng. J., 105, 144 (2016)

[6] Croissant JG, Fatieiev Y, Almalik A, Khashab NM, Adv. Healthc. Mater., 7 (2018)

[7] Xu ZG, Ma XQ, Gao YE, Hou ML, Xue P, Li CM, Kang YJ, Mater. Chem. Front., 1, 1257 (2017)

[8] Kane AL, Al-Shayeb B, Holec PV, Rajan S, Le Mieux NE, Heinsch SC, Psarska S, Aukema KG, Sarkar CA, Nater EA, Gralnick JA, Plos One, 11 (2016)

[9] Peng F, Su YY, Zhong YL, Fan CH, Lee ST, He Y, Accounts Chem. Res., 47, 612 (2014)

[10] Rahman IA, Padavettan V, J. Nanomater, 2012, 15 (2012)

[11] Kozlecki T, Polowczyk I, Bastrzyk A, Sawinski W, J. Nanopart, 2016, 9 (2016)

[12] Lin CH, Chang JH, Yeh YQ, Wu SH, Liu YH, Mou CY, Nanoscale, 7, 9614 (2015)

[13] Shekar S, Sander M, Riehl RC, Smith AJ, Braumann A, Kraft M, Chem. Eng. Sci., 70, 54 (2012)

[14] Patwardhan SV, Chem. Commun., 47, 7567 (2011)

[15] Kroger N, Deutzmann R, Sumper M, Science, 286, 1129 (1999)

[16] Lechner CC, Becker CFW, Mar. Drugs, 13, 5297 (2015)

[17] Cha JN, Shimizu K, Zhou Y, Christiansen SC, Chmelka BF, Stucky GD, Morse DE, Proc. Natl. Acad. Sci., 96, 361 (1999)

[18] Povarova NV, Markina NM, Baranov MS, Barinov NA, Klinov DV, Kozhemyako VB, Lukyanov KA, Biochem. Biophys. Res. Commun., 495(2), 2066 (2018)

[19] Richthammer P, Bormel M, Brunner E, van Pee KH, Chembiochem, 12, 1362 (2011)

[20] Ki MR, Jang EK, Pack SP, Process Biochem., 49(1), 95 (2014)

[21] Wang S, Xue J, Zhao Y, Du M, Deng L, Xu H, Lu JR, Soft Matter, 10, 7623 (2014)

[22] Knecht MR, Wright DW, Chem. Commun., 3038 (2003).

[23] Song IW, Park H, Park JH, Kim H, Kim SH, Yi S, Jaworski J, Sang BI, Sci. Rep., 7 (2017)

[24] Yeo KB, Ki MR, Park KS, Pack SP, Process Biochem., 58, 193 (2017)

[25] Naik RR, Brott LL, Clarson SJ, Stone MO, J. Nanosci. Nanotechnol., 2, 95 (2002)

[26] Han W, MacEwan S, Chilkoti A, Lopez GP, Nanoscale, 7, 12038 (2015)

[27] Kim TG, An GS, Han JS, Hur JU, Park BG, Choi SC, J. Korean Ceram. Soc., 54, 49 (2017)

[28] Xun L, Zhang L, Xing F, Lin H, Microporous Mesoporous Mater., 225, 238 (2016)

[29] Ki MR, Nguyen TKM, Jun HS, Pack SP, Process Biochem., 68, 182 (2018)

[30] Gao J, Maruyama A, Biohybrid Materials, Springer Berlin Heidelberg, Berlin, Heidelberg pp.1-5 2014.

[31] Kramer RM, Li C, Carter DC, Stone MO, Naik RR, J. Am. Chem. Soc., 126(41), 13282 (2004)

[32] Meldrum FC, Wade VJ, Nimmo DL, Heywood BR, Mann S, Nature, 349, 684 (1991)

[33] Ueno T, Suzuki M, Goto T, Matsumoto T, Nagayama K, Watanabe Y, Angew. Chem.-Int. Edit., 43, 2527 (2004)

[34] Lin X, Xie J, Niu G, Zhang F, Gao H, Yang M, Quan Q, Aronova MA, Zhang G, Lee S, Leapman R, Chen X, Nano Lett., 11, 814 (2011)

[35] Uchida M, Flenniken ML, Allen M, Willits DA, Crowley BE, Brumfield S, Willis AF, Jackiw L, Jutila M, Young MJ, Douglas T, J. Am. Chem. Soc., 128(51), 16626 (2006)

[36] Zhen Z, Tang W, Chen H, Lin X, Todd T, Wang G, Cowger T, Chen X, Xie J, ACS Nano, 7, 4830 (2013)

[37] Blazkova I, Nguyen HV, Dostalova S, Kopel P, Stanisavljevic M, Vaculovicova M, Stiborova M, Eckschlager T, Kizek R, Adam V, Int. J. Mol. Sci., 14(7), 13391 (2013)

[38] Clergeaud G, Genc R, Ortiz M, O'Sullivan CK, Langmuir, 29(49), 15405 (2013)

[39] Brunauer S, Emmett PH, Teller E, J. Am. Chem. Soc., 60, 309 (1938)

[40] Wieneke R, Bernecker A, Riedel R, Sumper M, Steinem C, Geyer A, Org. Biomol. Chem., 9, 5482 (2011)

[41] Strickland JDH, Parsons TR, A Practical Handbook of Seawater Analysis, Fisheries Research Board of Canada, 1972.

[42] Li X, Zhang JX, Gu HC, Langmuir, 27(10), 6099 (2011)

[43] Lambert EM, Viravaidya C, Li M, Mann S, Angew. Chem.-Int. Edit., 49, 4100 (2010)

[44] Yamada H, Urata C, Ujiie H, Yamauchi Y, Kuroda K, Nanoscale, 5, 6145 (2013)

[45] Kim M, Rho Y, Jin KS, Ahn B, Jung S, Kim H, Ree M, Biomacromolecules, 12(5), 1629 (2011)