Fed-batch acetone-butanol-ethanol fermentation using immobilized Clostridium acetobutylicum in calcium alginate beads

Russel Navarro Menchavez; Sung Ho Ha

Korean Journal of Chemical Engineering, Vol.36, No.6, 909-913, June, 2019

DOI10.1007/s11814-018-0232-z Export Citation

Fed-batch acetone-butanol-ethanol fermentation using immobilized Clostridium acetobutylicum in calcium alginate beads

Russel Navarro Menchavez, and Sung Ho Ha^{1, †}

Department of Biological Engineering, Inha University, Incheon 22212, Korea
¹Department of Advanced Materials & Chemical Engineering, Hannam University, Daejeon 34054, Korea

E-mail:

Butanol fermentation has garnered renewed interest due to the search for renewable sources of energy. Although acetone-butanol-ethanol (ABE) fermentation has been studied for a long time, the methods utilized yield either a high butanol/ABE productivity and low titer or high butanol/ABE titer at a low productivity. In this work, we report the utilization of a highly dense calcium alginate immobilized Clostridium acetobutylicum cells in combination with fed-batch mode of fermentation to attain a high butanol/ABE productivity and concentration. A butanol concentration of up to 21.6 g/L was attained with a productivity of 0.40 g/L·hr, which is a 65% and 192% improvement to the conventional batch fermentation.

Keywords:Butanol;ABE Fermentation;Fed-batch Fermentation;Immobilized C. acetobutylicum Cells

[References]

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[1] Garcia V, Pakkila J, Ojamo H, Muurinen E, Keiski RL, Energy Rev., 15, 964 (2011)

[2] Ndaba B, Chiyanzu I, Marx S, Biotechnol. Rep., 8, 1 (2015)

[3] Lee SY, Park JH, Jang SH, Nielsen LK, Kim J, Jung KS, Biotechnol. Bioeng., 101(2), 209 (2008)

[4] Trindade WRDS, Santos RGD, Renew. Sust. Energ. Rev., 69, 642 (2017)

[5] Sauer M, FEMS Microbiol. Lett., 363, fnw134 (2016)

[6] Xue C, Zhang X, Wang J, Xiao M, Chen L, Bai F, Biotechnol. Biofuels, 10, 148 (2017)

[7] Jones DT, Woods DR, Microbiol. Rev., 50, 484 (1986)

[8] Mariano AP, Filho RM, Bioenerg. Res., 5, 504 (2012)

[9] Outram V, Lalander CA, Lee JGM, Davies ET, Harvey AP, Biotechnol. Prog., 33(3), 563 (2017)

[10] Durre P, Biotechnol. J., 2, 1525 (2007)

[11] Jang YS, Lee JY, Lee J, Park JH, Im JA, Eom MH, Lee J, Lee SH, Song H, Cho JH, Seung DY, Lee SY, mBio, 3, e00314 (2012)

[12] Herman NA, Li J, Bedi R, Turchi B, Liu X, Miller MJ, Zhang W, Appl. Environ. Microbiol., 83, e02942 (2017)

[13] Sarchami T, Munch G, Johnson E, Kießlich S, Rehmann L, Fermentation, 2, 13 (2016)

[14] Zhu C, Chen LJ, Xue C, Bai FW, Biotechnol. Biofuels, 11, 128 (2018)

[15] Li SY, Chiang CJ, Tseng IT, He CR, Chao YP, FEMS Microbiol. Lett., 363, fnw107 (2016)

[16] Allam RF, Shafei MS, Sallam LAR, Egyp. J. Biomed. Sci., 8, 104 (2001)

[17] Sallam LAR, El-Refai AH, El-Zanati AE, Shafei MS, El-Ardi OAM, Arab J. Biotech., 7, 1 (2004)

[18] Chang Z, Cai D, Wang Y, Chen CJ, Fu CH, Wang GQ, Qin PY, Wang Z, Tan TW, Bioresour. Technol., 205, 82 (2016)

[19] Jeon YJ, Lee YY, Ann. N.Y. Acad. Sci., 506, 536 (1987)

[20] Miller GL, Anal. Chem., 31, 426 (1959)

[21] Ha SH, Mai NL, Koo YM, Process Biochem., 45(12), 1899 (2010)

[22] Menchavez RN, Ha SH, Appl. Biochem. Biotechnol., 171(5), 1159 (2013)

[23] Doremus MG, Linden JC, Moreira AR, Biotechnol. Bioeng., 27, 852 (1985)

[24] Lamed RJ, Lobos JH, Su TM, Appl. Environ. Microbiol., 54, 1216 (1988)

[25] Xia ML, Wang L, Yang ZX, Chen HZ, Biotechnol. Biofuels, 8, 225 (2015)

[26] Alsaker KV, Papoutsakis ET, J. Bacteriol., 187, 7103 (2005)

[27] Jones DT, Van Der Westhuizen A, Long S, Allcock ER, Reid SJ, Woods DR, Appl. Environ. Microbiol., 43, 1434 (1982)

[28] Chang YH, Chang KS, Chen CY, Hsu CL, Chang TC, Jang HD, Fermentation, 4, 45 (2018)

[29] Quiros C, Garcia LA, Diaz M, Process Biochem., 31(8), 813 (1996)

[30] Jain D, Bar-Shalom D, Drug Dev. Ind. Pharm., 40, 1576 (2014)

[31] Josef E, Zilberman M, Bianco-Peled H, Acta Biomater., 6, 4642 (2010)

[32] Yerushalmi L, Volesky B, Szczesny T, Appl. Microbiol. Biotechnol., 22, 103 (1985)

[33] Al-Shorgani NKN, Shukor H, Abdeshahian P, Kalil MS, Yusoff WMW, Hamid AA, Saudi J. Biol. Sci., 25, 1308 (2018)

[34] Ezeji TC, Qureshi N, Blaschek HP, Appl. Microbiol. Biotechnol., 63(6), 653 (2004)

[35] Qureshi N, Blaschek HP, Appl. Biochem. Biotechnol., 84, 225 (2000)

[36] Yu M, Du Y, Jiang W, Chang WL, Yang ST, Tang IC, Appl. Microbiol. Biotechnol., 93, 881 (2012)

[37] Choi YJ, Lee J, Jang YS, Lee SY, mBio, 5, e01524 (2014)