화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.74, 126-135, June, 2019
DOI10.1016/j.jiec.2019.02.017  Export Citation
Exosome-based antisense locked nucleic acid delivery for inhibition of type II collagen degradation in chondrocyte
Kyeongsoo Jeong1, Seunga Jeong1, Jeong Ah Kim2, and Won Jong Rhee1, 3, †
  • 1Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 406-772, Republic of Korea
  • 2Biomedical Omics Group, Korea Basic Science Institute, Cheongju, 28119, Republic of Korea
  • 3Division of Bioengineering, Incheon National University, Incheon, 406-772, Republic of Korea
E-mail:
MicroRNA-34a (miR-34a) suppression can block the progression of type II collagen decrease in chondrocytes for osteoarthritis (OA) treatment. Thus, exosomes, nano-sized extracellular vesicles, were developed as delivery vehicles for antisense miR-34a locked nucleic acid (LNA). As a result, antisense LNA-loaded exosomes exhibited no cytotoxic effects on the chondrocytes and inhibited IL-1β-mediated type II collagen decrease in inflammation-induced chondrocytes. Moreover, the suppression of chondrocyte growth was inhibited upon antisense LNA-loaded exosome treatment. In conclusion, the exosome-mediated antisense delivery approach was shown to be biocompatible and functional as a highly efficient OA treatment and could be applicable for treating other diseases.
Keywords:MicroRNA;Osteoarthritis;Antisense locked nucleic acid;Exosome;Delivery vehicle
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