In this study, the use of recombinant rhamnogalacturonan lyase CtRGLf in bio-scouring of cotton and enzymatic degumming jute was evaluated. The best concentration for bio-scouring of cotton and degumming of jute was found to be 12mg/mL (3.2U/mL) of crude CtRGLf. Cotton fabric treated with 12mg/mL (3.2U/mL) of crude CtRGLf took 30s to absorb a drop of water while the CtRGLf untreated fabric took 40min. The crude CtRGLf reduced the water absorption time of cotton fabric by 98% after 45min of treatment. FESEM images showed that crude CtRGLf (12mg/mL; 3.2U/mL), effectively removed the waxy compounds from the surface of jute fibres after 1h of treatment. These results showed that CtRGLf is a potentially important enzyme for the textile industry.