In this paper, we uniquely introduce a series of microscope objectives (MOs) combined with a polar interferometer for imaging individual nanoparticles in real-time. The MOs with cascade magnifications are used to magnify the individual nanoparticle interferogram in the entire field of the CCD camera. Single shot, interferogram is captured and then reconstructed by the angular spectrum method. Size of the individual nanoparticle is then calculated by a simple geometric optical equation. Using this technique, important applications in drug delivery that require precision in size and shape of nanocarriers can be demonstrated. Moreover, using this technology, biological samples with birefringence can be investigated precisely.