Preparation and characterization of a pH and thermosensitive linear L,D-octapeptide-poly(dimethylamino ethyl methacrylate) ((L-Val-D-Val)(4)-PDMAEMA) conjugate is reported. The hydrophobic uncharged linear (L-Val-D-Val)(4) octapeptide was designed to self-assemble in nanotubes by exploiting the tubular self assembling properties of linear peptides with regularly alternating enantiomeric sequences. pH and thermosensitive PDMAEMA was obtained by atom transfer radical polymerization (ATRP). The conjugate was prepared by click-chemistry on the solid phase synthetized peptide. Because of the strong interactions between the peptide moieties, long single channel nanotubes (0.2-1.5 mu m) are formed also at acidic pH with the fully charged polymer. At 25 degrees C and basic pH the size of the nanotubes did not change significantly. In basic conditions and temperature above the PDMAEMA lower critical solution temperature (LCST) a significant increase of the length of the nanotubes up to several micrometers is observed. The size is retained for several days after cooling back to room temperature. Sonication significantly reduces the nanotube length (0.2-0.5 mu m) forming low polydisperse nanotubes. The elongation of the nanotubes is fully reversible by restoring acidic pH. This is the first example, to our knowledge, of thermosensitive peptide-polymer single channel nanotubes with length that can be varied from hundreds of nanometers to several micrometers. (C) 2019 Elsevier Inc. All rights reserved.