Hypothesis: Ultrasensitive detections with enrichment approaches based on hydrophilic-hydrophobic patterns have attracted increasing attention in the early diagnosis and treatment of diseases. However, most of these techniques involve complicated micro-fabrications and chemical modifications to achieve their specific pattern substrate wettability. Hence, the development of a simple and effective approach for the construction of new surface wettability techniques for ultrasensitive detection is with great expectations. Experiments: We present a simple approach to fabricate the superwettable colloidal crystal (CC) micropatterns on superhydrophobic Morpho butterfly wing surface for the ultrasensitive detection. The superwettable CC micropatterns were easily obtained by infiltrating and self-assembling monodispersed silica colloidal nanoparticles on the plasma treated butterfly wing patterns. The analytes could be enriched onto the hydrophilic CC area due to the wettability difference between the hydrophilic CC area and the superhydrophobic substrate. Findings: It was demonstrated that the detection limit of thrombin was down to 1.8 x 10(-13) mol L-1 based on the fluorophore-labeled aptamer. Moreover, with two-dimensional position codes of these CC micropatterns for different probes, the multiplex detection capability was also demonstrated with great accuracy. As the elimination of complex instruments and chemical modifications, this proposed platform offers a simple strategy for ultrasensitive multiplex detection in practical applications. (C) 2019 Elsevier Inc. All rights reserved.