Triclosan is an important emerging pollutant. It has become ubiquitous due to its incomplete elimination in municipal wastewater treatment plants causing serious environmental problems. Biomass from microorganisms as sorbent of pollutants can be an eco-friendly alternative for triclosan removal. In this work, the elimination of triclosan using biomass (dead and living) of the marine microalga Phaeodactylum tricornutum was characterized in cultures exposed to light and in a complex solution (seawater). Maximum removal capacity, isotherms, kinetics, FTIR characterization, pH effect and reuse were evaluated and discussed. Photodegradation of triclosan was also evaluated. Both biomasses showed similar effectiveness; around 100% of pollutant was eliminated when its concentration was 1 mg L-1 in only 3 h using a biomass concentration of 0.4 g L-1. A pseudo-second order model guided the biosorption process. Considering the photodegradation as a first-order process, the whole process (photodegradation + biosorption) was suitably modelled with pseudo-third order and Elovich kinetics. Biosorption increased with the decrease in pH. Temkin isotherm showed the best fit for the experimental data. Both biomasses showed good reuse after five cycles, losing only 7% in efficiency. P. tricomutwn biomass is an attractive eco-material for triclosan elimination with low-cost and easy handling than other sorbents.