Developing a cost-effective electrocatalyst with high activity and excellent stability is very significant to nonenzymatic glucose detection. Herein, we have constructed 3D NiCo/NiCoOx nanocluster electrode modified by amorphous FeOOH nanosheets owing to the similar electrocatalytic activity of FeOOH to natural peroxidases. The NiCo nanoclusters can provide an excellent conductivity and high active surface areas, while the amorphous two-dimensional ultrathin structure of FeOOH stimulates more open metal sites for glucose oxidation. Besides, the strong synergetic effects among ternary Fe, Ni and Co are beneficial to the transport of charge. Though comprehensive electrochemical measurements, it is found that the as-prepared electrode possesses the prominent activity toward glucose oxidation with high sensitivity of 7138 mu AmM(-1)cm(-2), a wide linear range of 1 mu M and 8mM, a low detection limit of 0.82 mu M, good selectivity and excellent stability, all of which is mainly derived from the more active sites, rapid mass transport and superior electron transfer. This research provides a model system for developing more available electrocatalysts for glucose detection.