This work presents an approach to tailor the properties of the graphene oxide-silver nanoparticle (GO-AgNPs) composite using room temperature atmospheric plasma treatment. In particular, the aerosolized deposition of graphene oxide-silver nanoparticle composite (GO-AgNPs), the rapid reduction of GO at room temperature, and AgNPs surface excitation are investigated in this work. The plasma treatment of aerosolized GO leads to the reduced graphene oxide (rGO) formation which is observed from the increase in D to G band ratio from 0.65 for GO to 1.2 for rGO in the Raman spectra. Scanning Electron Microscopy, Transmission Electron Microscopy, and Selected Area Electron Diffraction patterns show that the plasma treatment leads to the morphological changes and the Electrochemical Impedance spectroscopy results show the improvement in the conductivity of the rGO-AgNP composite. To demonstrate the efficacy of the technique, plasma treated GO and silver nanoparticles (AgNPs) composite is used for the electrode surface modification of the commercial screen-printed electrodes for the cortisol detection. The cyclic voltammetry scans to detect cortisol shows that the sensitivity of the surface modified electrodes is increased after plasma treatment. This room temperature atmospheric plasma annealing technique is of specific interest for rapid processing of nanoparticles on flexible surfaces without subjecting them to elevated temperatures. (c) The Author(s) 2019. Published by ECS. This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, please email: [email protected].