Three stochastic microsensors designed using matrices based on diamond, graphite and graphene decorated with Pt nanoparticles modified with 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and protoporphyrin IX (PIX) were designed, characterized and validated for the assay of: C-reactive protein, adiponectin and Zn2+. The working concentration ranges were for C-reactive protein: 2.04 x 10(-8)-1.00mg/mL when the stochastic microsensor based on 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and graphite was used, 8.20 x 10(-8)-0.52mg/mL when the stochastic microsensor based on 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and diamond paste was used, 1.64 x 10(-8)-0.41mg/mL when the stochastic microsensor based on protoporphyrin IX and graphene decorated with Pt nanoparticles was used, for adiponectin 2.50 x 10(-8)-1.00 mu g/mL when the stochastic microsensor based on 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and graphite was used, 2.50 x 10(-8)-0.25 mu g/mL when the stochastic microsensor based on 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and diamond paste was used, 2.50 x 10(-8)-0.25 mu g/mL when the stochastic microsensor based on protoporphyrin IX and graphene decorated with Pt nanoparticles was used, and for Zn2+: 1.36 x 10(-10)-1.00 mu g/mL when the stochastic microsensor based on 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and graphite was used, 1.36 x 10(-8)-1.00 mu g/mL when the stochastic microsensor based on 2,6-bis (E)-2-(thiophen-3-yl)-4-4-(4,6,8-trimethylazulen-1-1yl) pyridine and diamond paste was used, 1.36 x 10(-8)-1.00 mu g/mL when the stochastic microsensor based on protoporphyrin IX and graphene decorated with Pt nanoparticles was used. Very low limits of determination were recorded. The sensitivities of all tested stochastic microsensors were very high. The validation of the screening method for serum samples was done, high accuracy and precision being recorded. (C) The Author(s) 2019. Published by ECS.