Precise synthesis of polymer particles with topology and surface chemistry spanning from isotropy to anisotropy is critically needed for the fundamental research and industrial production. Herein, we report a seed wettability-manipulated emulsion interfacial polymerization to precisely synthesize polymer particles spanning from anisotropic Janus particles to spherical heterogeneous nano porous particles. The variation of seed wettability from hydrophobicity to hydrophilicity allows a topological transition of obtained polymer particles from anisotropic Janus particles to spherical heterogeneous nanoporous particles, along with the distribution of surface chemistry. This large-span evolution of topology and surface chemistry of obtained particles is due to the shifting of the initially polymerizable emulsion interface from oil-in-water (O/W) to water-in-oil-in-water (W/O/W) driven by osmotic pressure. This seed wettability-manipulated emulsion interfacial polymerization is applicable to a variety of hydrophobic/hydrophilic monomer pairs, allowing flexible fabrication of diverse Janus particles and heterogeneous nanoporous particles.