The present research work is focused on the green synthesis of rutile titanium dioxide (GST) nanoparticles using leaf extract of piper nigrum plant. The synthesis yielded rutile GST with a particle size of 6.45 +/- 0.81 nm and a zeta potential value of -27.51 mV. Breath-figure method was implemented to fabricate honeycomb films and the porous honeycomb PLA/GST (H-PLA/GST) nanocomposites have shown tremendous improvement of antibacterial activity against S. aureus under visible light or in darkness. The highest antibacterial potentiality expressed in terms of zone of inhibition (ZOI) was exhibited by the H-PLA/3GST nanocomposites (28 mm in visible and no activity in dark). H-PLA/GST after cytocompatibility studies had seeded cells with flattened morphology, showing better cell adhesion.