Formation, growth, and detachment of injected air bubble from a submerged needle in stagnant liquid at the different temperature of fluid, investigated numerically. Experiments have been done for validation of numerical simulation results. The injection flow rate of air was varied between 600 and 1200 mL/h in experimental study. Bubble formation, growth, and detachment information were obtained using high speed camera and visual photography technique. Young-Laplace equation that was derived from the force balance on the bubble, was utilized in numerical simulation. A novel method was used for solution of Young-Laplace equation. The bubble diameter, instantaneous contact angle, volume, and other characteristics of bubble were studied at different temperature of operating condition. Also, the enhancement of temperature in Al2O3 nanofluid with 0.01% volume concentration (phi = 0.01%) was compared with deionized water results. The results reveal that by increasing the Al2O3 nanofluid and the deionized water temperature, the diameter, volume, and center of gravity of bubble decrease; however, the instantaneous contact angle increases. Meanwhile, the size of bubble at Al2O3 nanofluid is larger than of that in deionized water at same temperature. Also, the bubble aspect ratio is almost senseless to temperature increment in both deionized water and Al2O3 nanofluid. Eventually, the variation of operating temperature and adding of nanoparticle to the deionized water have significant influence on behavior of growing bubble.