Distillation processes for separation of biphasic ABE solution from in sins gas stripping were developed. The distillation sequences were investigated and optimized. Results proved the energy requirement of distillation was effectively reduced when feeding the biphasic ABE mixture. After techno-economic analysis, a superior scenario that started with a decanter followed with side-by-side sequences for separation acetone and ethanol was selected. Compared with the conventional processes that detractively concentrate the biphasic ABE mixture, energy requirement and total annual cost (TAC) of the novel distillation process were decreased by 25.8% and 17.4%, respectively. $ 4102.98 K per year of TAC was estimated in a case with a capacity of 30 K tons of butanol per year.