Phosphate-Functionalized Stabilized F127 Nanoparticles: Introduction of Discrete Surface Charges and Electrophoretic Determination of Aggregation Number

Juyoung Hwang; Mingyeong Kang; Mila Irva Sari; Tae Hyeong Lee; Kibeom Kim; Yeol Kyo Choi; Eunji Lee; Ja-Hyoung Ryu; Seung Ki Baek; Dong June Ahn; Peter C. W. Lee; Minseok Kwak

Macromolecular Research, Vol.27, No.7, 657-662, July, 2019

DOI10.1007/s13233-019-7100-x Export Citation

Phosphate-Functionalized Stabilized F127 Nanoparticles: Introduction of Discrete Surface Charges and Electrophoretic Determination of Aggregation Number

Juyoung Hwang, Mingyeong Kang, Mila Irva Sari, Tae Hyeong Lee¹, Kibeom Kim², Yeol Kyo Choi³, Eunji Lee⁴, Ja-Hyoung Ryu², Seung Ki Baek⁵, Dong June Ahn³, Peter C. W. Lee^{1, †}, and Minseok Kwak^†

Department of Chemistry, Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Korea
¹Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea
²Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea
³Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Korea
⁴School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea
⁵Department of Physics, Pukyong National University, Busan 48513, Korea

E-mail:,

Pluronic F127 forms spherical micelle with a defined number of molecules aggregated in aqueous environments. Such self-assembled micelles dissociate into unimers below certain concentration and temperature. We stabilized the micelles by semi-interpenetrating network (sIPN) formation within the hydrophobic core in the presence of a fluorescent dye. Additionally, by varying the mixing ratio of negatively charged and pristine F127s we prepared thermally stable polymeric nanoparticles with discrete surface charges within nearly same sizes. Using the nanomaterials with prescribed number of charges, we demonstrate that the electrophoretic mobility of nanoparticles is solely depending on number of surface charges. Finally, the aggregation number (Nagg) of F127 was further determined by electrophoresis.

Keywords:self-assembly;encapsulation;polymer aggregates;Pluronic;sIPNs

[References]

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Kim CH, Kim SY, Lim YT, Lee TS, Macromol. Res., 25(6), 572 (2017)
Kabanov AV, Batrakova EV, Alakhov VY, J. Control. Release, 82, 189 (2002)
Singh A, Kim W, Kim Y, Jeong K, Kang CS, Kim Y, Koh J, Mahajan SD, Prasad PN, Kim S, Adv. Funct. Mater., 26(39), 7057 (2016)
Chaibundit C, Ricardo GMPS, Costa VDMLL, Yeates SG, Booth C, Langmuir, 23(18), 9229 (2007)
Hong KH, Jeon YS, Kim JH, Macromol. Res., 17(1), 26 (2009)
Su YL, Wang J, Liu HZ, Macromolecules, 35(16), 6426 (2002)
Yani C, Bredenkamp MW, Jacobs EP, Swart P, Bioorg. Med. Chem. Lett., 13, 1381 (2003)
Alexandridis P, Holzwarth JF, Hatton TA, Macromolecules, 27(9), 2414 (1994)
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Higuchi A, Yamamoto T, Sugiyama K, Hayashi S, Tak TM, Nakagawa T, Biomacromolecules, 6(2), 691 (2005)
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Zaborin A, Defazio JR, Kade M, Kaiser BLD, Belogortseva N, Camp DG, Smith RD, Adkins JN, Kim SM, Alverdy A, Antimicrob. Agents Chemother., 58, 966 (2014)
Lindahl E, Hess B, Van Der Spoel D, J. Mol. Model, 7, 306 (2001)
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[2] Kim CH, Kim SY, Lim YT, Lee TS, Macromol. Res., 25(6), 572 (2017)

[3] Kabanov AV, Batrakova EV, Alakhov VY, J. Control. Release, 82, 189 (2002)

[4] Singh A, Kim W, Kim Y, Jeong K, Kang CS, Kim Y, Koh J, Mahajan SD, Prasad PN, Kim S, Adv. Funct. Mater., 26(39), 7057 (2016)

[5] Chaibundit C, Ricardo GMPS, Costa VDMLL, Yeates SG, Booth C, Langmuir, 23(18), 9229 (2007)

[6] Hong KH, Jeon YS, Kim JH, Macromol. Res., 17(1), 26 (2009)

[7] Su YL, Wang J, Liu HZ, Macromolecules, 35(16), 6426 (2002)

[8] Yani C, Bredenkamp MW, Jacobs EP, Swart P, Bioorg. Med. Chem. Lett., 13, 1381 (2003)

[9] Alexandridis P, Holzwarth JF, Hatton TA, Macromolecules, 27(9), 2414 (1994)

[10] Kabanov AV, Nazarova IR, Astafieva IV, Batrakova EV, Alakhov VY, Yaroslavov AA, Kabanov VA, Macromolecules, 28(7), 2303 (1995)

[11] Tummino PJ, Gafni A, Biophys. J., 64, 1580 (1993)

[12] Sharma PK, Bhatia SR, Int. J. Pharm., 278, 361 (2004)

[13] Higuchi A, Yamamoto T, Sugiyama K, Hayashi S, Tak TM, Nakagawa T, Biomacromolecules, 6(2), 691 (2005)

[14] Wu CH, Liu TB, Chu BJ, Schneider DK, Graziano V, Macromolecules, 30(16), 4574 (1997)

[15] Attwood D, Collett J, Tait C, Int. J. Pharm., 26, 25 (1985)

[16] Zaborin A, Defazio JR, Kade M, Kaiser BLD, Belogortseva N, Camp DG, Smith RD, Adkins JN, Kim SM, Alverdy A, Antimicrob. Agents Chemother., 58, 966 (2014)

[17] Lindahl E, Hess B, Van Der Spoel D, J. Mol. Model, 7, 306 (2001)

[18] Van Der Spoel E, Lindahl E, Hess B, Groenhof G, Mark AE, Berendsen HJ, J. Comput. Chem., 26, 1701 (2005)

[19] Hess B, Kutzner C, Van Der Spoel D, Lindahl E, J. Chem. Theory Comput., 4, 435 (2008)

[20] Marrink SJ, de Vries AH, Mark AE, J. Phys. Chem. B, 108(2), 750 (2004)

[21] Marrink SJ, Risselada HJ, Yefimov S, Tieleman DP, de Vries AH, J. Phys. Chem. B, 111(27), 7812 (2007)

[22] Lee H, de Vries AH, Marrink SJ, Pastor RW, J. Phys. Chem. B, 113(40), 13186 (2009)

[23] Uusitalo JJ, Ingolfsson HI, Akhskhi P, Tieleman DP, Marrink SJ, J. Chem. Theory Comput, 11, 3932 (2015)

[24] Hezaveh S, Samanta S, De Nicola A, Milano G, Roccatano D, J. Phys. Chem. B, 16, 14333 (2012)

[25] Bussi G, Donadio D, Parrinello M, J. Chem. Phys., 126, 014101 (2007)

[26] Berendsen HJ, Postma JV, van Gunsteren WF, Dinola A, Haak J, J. Chem. Phys., 81, 3684 (1984)

[27] Parrinello M, Rahman A, J. Appl. Phys., 52, 7182 (1981)

[28] Martinez L, Andrade R, Birgin EG, Martinez JM, J. Comput. Chem., 30, 2157 (2009)

[29] Petrov P, Bozukov M, Burkhardt M, Muthukrishnan S, Muller AH, Tsvetanov CB, J. Mater. Chem., 16, 2192 (2006)

[30] Petrov P, Bozukov M, Tsvetanov CB, J. Mater. Chem., 15, 1481 (2005)

[31] Abdi H, in Encyclopedia of Measurement and Statistics, Sage, Thousand Oaks, CA, p 508 2007.

[32] Kwak M, Musser AJ, Lee J, Herrmann A, Chem. Commun., 46, 4935 (2010)