A novel hybrid interface of photo-reduced graphene oxide on TiO2 nanotube (TiNT) matrix was developed as a sensing electrode for L-Tyrosine detection towards the development of smart bandage for wound monitoring application, specifically for diabetic foot ulcer (DFUs) patients. The TiNT matrix was developed on titanium (Ti) foil using electrochemical anodization, further; graphene oxide (GO) thin film was electrodeposited on it. This hybrid nanostructure was then photo-reduced using UV light (lambda = -290 nm) for 4 min, leading to the conversion of GO to reduced graphene oxide (rGO). The TiNT-rGO hybrid electrode thus formed was further modified with Tyrosinase enzyme for selective detection of tyrosine, which is a bio-marker for DFUs. The sensitivity and limit of detection (LoD) of the developed sensor was found to be 0.106 Omega/nM/cm(2) and 0.35 nM respectively, with response time of < 1 min. The proposed sensor was also tested at different pH and temperature conditions to model real-time wound conditions. The modified electrode also demonstrated good stability and reproducibility with a shelf life of nearly 30 days.