In this work, TiO2:Co thin films were prepared by the DC Magnetron Co-Sputtering system on glass substrates, varying the substrate temperature. The magnetron power for each target (TiO2, Co), work pressure and deposition time in the samples were kept constant. From XRD, XPS and EDXS measurements, it was possible to establish the formation of a TiO2 semiconductor matrix without binary phases of cobalt oxides. AFM and MFM micrographs evidenced the formation of smaller grains without domains in the surface. The magnetic curves show the dependence of the substrate temperature in the magnetic behaviour of thin films. The presences of hysteresis loops at high temperature in the thin films were observed.