Herein, we compared the catalyst formation process of three Pt/TiO2 catalysts prepared by impregnation (PtTi-I), deposition precipitation (PtTi-D) and photo-deposition (PtTi-P) to investigate its effect on oxgyen activation and toluene oxidation activity. The encapsulation of TiO2 on Pt particles induced by high temperature strengthened the metal-support interaction (MSI) but inhibited the activation of oxygen over PtTi-I catalyst, thus it showed the poorest activity in toluene oxidation. Since the selective deposition of Pt on certain face of TiO2 during photo-deposition process, PtTi-P exhibited stronger MSI and higher activity although it possessed much larger Pt particles than PtTi-D.