The Ni/SBA-15 and Ni/TiO2 catalysts with 20 wt% nickel with different metal-support interaction strength were designed using ordered mesoporous silica (SBA-15) and TiO2 (P25). The Ni/SBA-15 and Ni/TiO 2 catalysts with 20 wt% nickel were investigated using various characterization techniques. It was found that the Ni/SBA-15 and Ni/TiO2 catalysts with 20 wt% nickel possess different nickel particle size and different reducibility because of the different strength between nickel and support interaction. Both Ni/SBA-15 and Ni/TiO2 catalysts were tested for the hydrodeoxygenation reaction of a model bio-oil using a fixed-bed reactor at different evaluation conditions. It was found that under high hydrogen pressure (>= 7 bar), both Ni/SBA-15 and Ni/TiO2 catalysts are highly efficient because the organic oxygen compound could be converted completely. And the product selectivity is very sensitive to the hydrogen pressure. Under low hydrogen pressure (3-5 bar) Ni/TiO2 catalyst show rather higher selectivity toward aromatic hydrocarbon as compared with Ni/SBA-15 catalyst. Lower hydrogen pressure and reaction temperature will be beneficial to the aromatic hydrocarbon production. It is very important to reduction for the H-2 consumption cost. TiO2 is a very potential candidate support for the catalyst design aiming to the sustainable aromatic hydrocarbon production.