Designing and fabricating highly active and efficient catalysts are of vital importance for the practical applications of direct ethylene glycol fuel cells (DEGFCs). In this study, we employ a feasible one-pot synthetic method to construct highly monodispersed PtCu nanospheres (NSs) as high-efficiency anode electrocatalysts for DEGFCs. Interestingly, the optimized carbon supported Pt1Cu1 NSs can display the highest mass activity of 2146.9 mA mg(-1) in 1 M KOH + 1 M EG solution under the scan rate of 50 mV s(-1), which is 1.9 times higher than that of commercial Pt/C catalysts. This is ascribed to the favorable electronic effect between Pt and Cu, which is beneficial for ethylene glycol oxidation reaction (EGOR) in fuel cells. Meanwhile, such monodispersed NSs can also exhibit excellent durability, where the Pt1Cu1 catalyst retains 62.6% of the initial value after the cyclic voltammetry of 500 cycles. This work not only provides a significant approach for designing catalysts for fuel cells, but also constructs a novel class of active and stable electrocatalysts for EGOR. (C) 2019 Published by Elsevier Inc.